e-infrastructure Roadmap for Open Science in Agriculture

A bibliometric study

The e-ROSA project seeks to build a shared vision of a future sustainable e-infrastructure for research and education in agriculture in order to promote Open Science in this field and as such contribute to addressing related societal challenges. In order to achieve this goal, e-ROSA’s first objective is to bring together the relevant scientific communities and stakeholders and engage them in the process of coelaboration of an ambitious, practical roadmap that provides the basis for the design and implementation of such an e-infrastructure in the years to come.

This website highlights the results of a bibliometric analysis conducted at a global scale in order to identify key scientists and associated research performing organisations (e.g. public research institutes, universities, Research & Development departments of private companies) that work in the field of agricultural data sources and services. If you have any comment or feedback on the bibliometric study, please use the online form.

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Title

Transcript Profiling in Arabidopsis with Genome Tiling Microarrays

en
Abstract

Microarray technology is at present a standardized workflow for genome-wide expression analysis. Whole-genome tiling microarrays have emerged as an important platform for flexible and comprehensive expression profiling. In this chapter we describe a detailed standardized workflow for experiments assessing the transcriptome of Arabidopsis using tiling arrays and provide useful hints for critical steps from experimental design to data analysis. Although the protocol is optimized for AGRONOMICS1 arrays, it can readily be adapted to other tiling arrays. AGRONOMICS1 is the first platform that enables strand-specific expression analysis of the Arabidopsis genome with a single array. Moreover, it includes all perfect match probes from the original ATH1 array, allowing readily integration with the large existing ATH1 knowledge base. This workflow is designed for the analysis of raw data for any number of samples and it does not pose any particular hardware requirements.

en
Year
2013
en
Country
  • CH
  • SE
Organization
  • ETH_Zurich_Swiss_Fed_Inst_Technol_Zurich (CH)
  • Swedish_Univ_Agr_Sci_SLU (SE)
  • Linnean_Ctr_Plant_Biol (SE)
Data keywords
  • knowledge
  • knowledge based
en
Agriculture keywords
    en
    Data topic
    • big data
    en
    SO
    TILING ARRAYS: METHODS AND PROTOCOLS
    Document type

    Inappropriate format for Document type, expected simple value but got array, please use list format

    Institutions 10 co-publis
    • ETH_Zurich_Swiss_Fed_Inst_Technol_Zurich (CH)
    • Swedish_Univ_Agr_Sci_SLU (SE)
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    e-ROSA - e-infrastructure Roadmap for Open Science in Agriculture has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 730988.
    Disclaimer: The sole responsibility of the material published in this website lies with the authors. The European Union is not responsible for any use that may be made of the information contained therein.