e-infrastructure Roadmap for Open Science in Agriculture

A bibliometric study

The e-ROSA project seeks to build a shared vision of a future sustainable e-infrastructure for research and education in agriculture in order to promote Open Science in this field and as such contribute to addressing related societal challenges. In order to achieve this goal, e-ROSA’s first objective is to bring together the relevant scientific communities and stakeholders and engage them in the process of coelaboration of an ambitious, practical roadmap that provides the basis for the design and implementation of such an e-infrastructure in the years to come.

This website highlights the results of a bibliometric analysis conducted at a global scale in order to identify key scientists and associated research performing organisations (e.g. public research institutes, universities, Research & Development departments of private companies) that work in the field of agricultural data sources and services. If you have any comment or feedback on the bibliometric study, please use the online form.

You can access and play with the graphs:

Discover all records
Home page

Title

DETERMINATION OF AFLATOXIN M1 IN MILK SAMPLES BY AN OWLS-BASED IMMUNOSENSOR

en
Abstract

An immunoreaction-based method was investigated for the detection of aflatoxin M1 (AFM1), which is the hydroxylated metabolite of aflatoxin B1 (AFB1). This mycotoxin may be found in milk and milk products obtained from livestock that have ingested contaminated feed. Quantitative analysis of AFM1 was carried out using indirect (competitive) immunoassay method, which can be used for low weight molecules. The real-time measurement was done with Optical Waveguide Lightmode Spectroscopy (OWLS) technique. After the optimization of the chemical and biochemical parameters (determination of the optimal concentration of the immobilized AFM1-protein conjugate, determination of the AFM1 antibody content of the samples, etc.) real samples were also examined. Three kinds of milk sample preparation methods (filtration, centrifugation, size exclusion centrifugation) and two dilution rates (100 and 200 fold) were compared. The presented competitive immunoassay method showed the best results when 100 fold diluted filtered or centrifuged milk samples were examined. The dynamic measuring ranges for AFM1 were 0.001-0.1 ng ml(-1) and 0.0005-0.01 ng ml(-1), respectively.

en
Year
2014
en
Country
  • HU
Organization
    Data keywords
      en
      Agriculture keywords
      • livestock
      en
      Data topic
      • semantics
      en
      SO
      ACTA ALIMENTARIA
      Document type

      Inappropriate format for Document type, expected simple value but got array, please use list format

      Institutions 10 co-publis
        uid:/V12RSRRC
        Powered by Lodex 8.20.3
        logo commission europeenne
        e-ROSA - e-infrastructure Roadmap for Open Science in Agriculture has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 730988.
        Disclaimer: The sole responsibility of the material published in this website lies with the authors. The European Union is not responsible for any use that may be made of the information contained therein.