e-infrastructure Roadmap for Open Science in Agriculture

A bibliometric study

The e-ROSA project seeks to build a shared vision of a future sustainable e-infrastructure for research and education in agriculture in order to promote Open Science in this field and as such contribute to addressing related societal challenges. In order to achieve this goal, e-ROSA’s first objective is to bring together the relevant scientific communities and stakeholders and engage them in the process of coelaboration of an ambitious, practical roadmap that provides the basis for the design and implementation of such an e-infrastructure in the years to come.

This website highlights the results of a bibliometric analysis conducted at a global scale in order to identify key scientists and associated research performing organisations (e.g. public research institutes, universities, Research & Development departments of private companies) that work in the field of agricultural data sources and services. If you have any comment or feedback on the bibliometric study, please use the online form.

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Rapid generation and analysis of expressed sequence tags to uncovering inflorescence secondary metabolism of Bougainvillea spectabilis 'Speciosas' by pyrosequencing


Bougainvilleas, are one of the unique plant families with an unusual class of pigments, known as betalains replaced the more common anthocyanins. However, little is known on the molecular mechanism on the mutual exclusion of anthocyanins and betalains. In order to explore the unique phenomena in Bougainvilleas, we used open inflorescences of B. spectabilis 'Speciosas' for transcriptome analysis by pyrosequencing, which is the first attempt to obtain the transcriptome of this specific plant. In this study, we obtained 111.9 M raw data, after assembling, 17,728 unigenes sequence with average length of 607 bp long were available, the annotation including Gene Ontology, COG/KOG and associated Kyoto Encyclopedia of Genes and Genomics (KEGG) pathway maps were conducted. Important genes involved in flavonoid/Betalains/anthocyanins motablisms were identified including FLS/F3H, UGT, DFR, DODA, anthocyanidin reductase and Anthocyanidin 3-O-glucosyltransferase, as well as some transcriptional factors (Myb, YABBY, MADS-box, F-box, WD-40 and bHLH) in this study. The obtained unigenes which will provide a database for discovering genes involved in secondary metabolic synthesis pathway. The discovered genes can be used for future bioengineering of various secondary metabolites for health improvement, pigments, medicine and agricultural production, etc. Specially, these genes such as DFR, anthocyanidin reductase and anthocyanidin 3-O-glucosyltransferase, were detected in betalain-synthesizing plants, which is exciting for researchers. Furthermore, a total of 877 simple sequence repeat motifs (SSRs) were identified, among the perfect SSRs, the most abundant repeat units were mononucleotide repeats (61 %) with (A/T)(n) and the second one is tri-nucleotide repeats (24 %). The obtained EST-SSRs can be used for marker development which will be beneficial for the studies in the aspects of genetic diversity, evolution and phenotype variation of B. spectabilis 'Speciosas' as well as its related species.

  • CN
    Data keywords
    • ontology
    Agriculture keywords
    • agriculture
    Data topic
    • information systems
    • semantics
    Document type

    Inappropriate format for Document type, expected simple value but got array, please use list format

    Institutions 10 co-publis
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      e-ROSA - e-infrastructure Roadmap for Open Science in Agriculture has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 730988.
      Disclaimer: The sole responsibility of the material published in this website lies with the authors. The European Union is not responsible for any use that may be made of the information contained therein.