e-infrastructure Roadmap for Open Science in Agriculture

A bibliometric study

The e-ROSA project seeks to build a shared vision of a future sustainable e-infrastructure for research and education in agriculture in order to promote Open Science in this field and as such contribute to addressing related societal challenges. In order to achieve this goal, e-ROSA’s first objective is to bring together the relevant scientific communities and stakeholders and engage them in the process of coelaboration of an ambitious, practical roadmap that provides the basis for the design and implementation of such an e-infrastructure in the years to come.

This website highlights the results of a bibliometric analysis conducted at a global scale in order to identify key scientists and associated research performing organisations (e.g. public research institutes, universities, Research & Development departments of private companies) that work in the field of agricultural data sources and services. If you have any comment or feedback on the bibliometric study, please use the online form.

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Generation of expressed sequence tags from carob (Ceratonia siliqua L.) flowers for gene identification and marker development


Carob (Ceratonia siliqua L.) is a caesalpinoid legume tree showing labile sex expression. With the main aims of identifying flower-expressed genes and of developing specific markers, 1,056 clones from a complementary DNA library of carob flowers were bidirectionally sequenced. A total of 1,377 high-quality expressed sequence tags were clustered into 1,096 unigenes. Basic Local Alignment Search Tool and Gene Ontology functional annotation allowed to identify several agronomically important genes, such as those involved in flower development and sexual reproduction, response to stress, galactomannan synthesis, and hormone pathways. Genes involved in the ethylene biosynthesis and response were quantified in developing flowers of three sex genotypes (male, female, and hermaphrodite) using quantitative reverse transcription polymerase chain reaction. The transcript levels of 1-aminocyclopropane-1-carboxylic acid oxidase, acting downstream in ethylene pathway, and Ethylene Insensitive 3 (EIN3)-like, a transcription factor involved in ethylene signaling, were directly correlated with maleness, indicating a possible role of ethylene in carob sex expression. Furthermore, the first set of carob genic microsatellites was developed, which might be useful for genotyping and genetic diversity analysis.

  • IT
  • US
  • Univ_Calif_Riverside (US)
  • Univ_Catania (IT)
Data keywords
  • ontology
Agriculture keywords
    Data topic
    • information systems
    • semantics
    Document type

    Inappropriate format for Document type, expected simple value but got array, please use list format

    Institutions 10 co-publis
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      e-ROSA - e-infrastructure Roadmap for Open Science in Agriculture has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 730988.
      Disclaimer: The sole responsibility of the material published in this website lies with the authors. The European Union is not responsible for any use that may be made of the information contained therein.